Illuminating GFP

Illuminating EM with GFP

The ability to use GFP-tagged proteins to perform correlative light and electron microscopy has been a desirable but elusive method. The high quantum yield of GFP that makes it excellent for fluorescence imaging also makes it extremely inefficient for production of the reactive oxygen needed to trigger formation of an electron-dense precipitate for electron microscopy. This roadblock has finall...

Illuminating Nature

At the base level of creative practice, artists often reap inspiration from their surroundings. Writers might eavesdrop on a conversation in a coffee shop and then use it in a novel, and a composer might transform sounds from his urban environment into an orchestral work. In Kelly Lanphier’s case, working at the Aquarium of the Pacific in Long Beach, California, early on created a substantial i...

Illuminating Illumination

We introduce an active imaging method to measure scene illumination. The system implementation is divided into four steps. First, the system acquires two images: one is an ordinary image of the scene under ambient light and the other is a corresponding image in which light from the camera flash is added to the scene. Second, the image pair is analyzed to obtain an image that represents the scen...

Illuminating the Sites of Enterovirus Replication in Living Cells by Using a Split-GFP-Tagged Viral Protein

Like all other positive-strand RNA viruses, enteroviruses generate new organelles (replication organelles [ROs]) with a unique protein and lipid composition on which they multiply their viral genome. Suitable tools for live-cell imaging of enterovirus ROs are currently unavailable, as recombinant enteroviruses that carry genes that encode RO-anchored viral proteins tagged with fluorescent repor...

Illuminating the Universe's Ignition